Ultrasound-Assisted Green Extraction of Flavonoid-Rich Biocompounds from Camellia Sinensis Leaves and Evaluation of Antioxidant Activity

Abstract

The present study investigated the ultrasound-assisted green extraction of Flavonoid-rich biocompounds from Camellia Sinensis leaves and evaluated their antioxidant activity. Ultrasound-Assisted Extraction (UAE) was employed as an environmentally friendly and efficient extraction technique to enhance the recovery of bioactive phytochemicals while reducing extraction time and solvent consumption. Different extraction times were examined to evaluate their effects on extraction yield, Total Phenolic Content (TPC), Total Flavonoid Content (TFC), and antioxidant activity. The obtained results demonstrated that increasing ultrasonication time significantly improved extraction efficiency and recovery of phenolic and Flavonoid compounds. The highest extraction yield, TPC, and TFC were observed at 30 min of extraction. Antioxidant activity was evaluated using the 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging assay, and the extracts exhibited considerable free radical scavenging activity that increased with increasing extraction time. The enhanced antioxidant activity was strongly associated with the elevated concentration of Flavonoids and polyphenolic compounds extracted from the green tea leaves. The findings of this study indicate that UAE is an effective green technology for the sustainable recovery of antioxidant-rich biocompounds from Camellia Sinensis. Furthermore, the obtained extracts demonstrate promising potential for applications in pharmaceutical, nutraceutical, food, and cosmetic industries due to their high antioxidant properties and natural bioactive composition.