Evaluation of Antioxidant Constituents and Cytotoxic Effects of Rumex Tuberosus Leaf Extract on Colorectal Cancer Cells

Authors

  • Mohammad Hassan Rostay Fallah Department of Microbiology, To.C., Islamic Azad University, Tonekabon, Iran.
  • Simin Aryan * Department of Microbiology, To.C., Islamic Azad University, Tonekabon, Iran.

https://doi.org/10.48313/bic.vi.33

Abstract

Colon cancer is one of the most common causes of mortality worldwide and has been identified as the second leading cause of cancer-related deaths among adults. Therefore, there is an urgent need to control this malignancy using therapeutic approaches with minimal side effects. The present study aimed to identify the antioxidant compounds in the leaf extract of (Rumex Tuberosus) and to evaluate its inhibitory effects on the growth of colorectal cancer cells. For this purpose, the leaves of R. Tuberosus were collected from Tonekabon, air-dried in the shade, and ground into powder. The extracts were prepared by maceration, and the total phenolic, flavonoid, and anthocyanin contents were determined. The HT29 colorectal cancer cell line was cultured and treated with various concentrations of the leaf extract (ranging from 62.5 to 2000 µg/ml) for 24, 48, and 72 hours. Cell viability and cytotoxicity were assessed using the MTT colorimetric assay. The results showed that the leaf extract of R. Tuberosus was rich in phenolic compounds, with the highest content of 17.19 ± 1.015 mg/g dry weight. Moreover, the MTT assay revealed a concentration- and time-dependent decrease in colorectal cancer cell viability, with the maximum inhibition (87.86%) observed at 2000 µg/ml after 72 hours of incubation. These findings suggest that the leaf extract of Rumex Tuberosus possesses notable anticancer properties and may serve as a promising candidate for further development in pharmaceutical applications.

Keywords:

Rumex Tuberosus, Antioxidant compounds, MTT assay, Colorectal cancer, Total phenolic content

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Published

2025-03-26

Issue

Vol. 2 No. 1 (2025): Biocompounds

Section

Articles

How to Cite

Rostay Fallah, M. H. ., & Aryan, S. . (2025). Evaluation of Antioxidant Constituents and Cytotoxic Effects of Rumex Tuberosus Leaf Extract on Colorectal Cancer Cells. Biocompounds, 2(1), 63-69. https://doi.org/10.48313/bic.vi.33