Assessment of Total Phenolic, Flavonoid, and Anthocyanin Contents of Amaranthus Retroflexus Extract and Its Inhibitory Effect on the Growth of Pseudomonas Aeruginosa

Authors

  • Pouria Moghadam Department of Microbiology, To.C., Islamic Azad University, Tonekabon, Iran.
  • Simin Aryan * Department of Microbiology, To.C., Islamic Azad University, Tonekabon, Iran.

https://doi.org/10.48313/bic.vi.80

Abstract

The use of medicinal plants in the treatment of bacterial infections has attracted increasing attention due to the growing resistance of bacteria to antibiotics and the adverse effects associated with their use. Therefore, this study aimed to determine the total Phenolic, Flavonoid, and Anthocyanin contents of Amaranthus Retroflexus extract and to evaluate its inhibitory effect on the growth of Pseudomonas aeruginosa. For this purpose, A. retroflexus plants were collected from an area near Tonekabon, Iran. The antioxidant compounds of the plant extract, including Phenolic, Flavonoid, and Anthocyanin compounds, were determined. In addition, the inhibitory effect of the extract on the growth of P. aeruginosa was evaluated at different concentrations. The results showed that the A. retroflexus extract was rich in antioxidant compounds, particularly Phenolic compounds, with a Total Phenolic Content (TPC) of 9.84±0.741 mg/g Dry Weight (DW). Furthermore, the Anthocyanin content of the extract was higher than its Flavonoid content. Regarding its antibacterial activity, the extract exhibited minimal inhibitory effects against P. aeruginosa at concentrations of 800 and 1000 mg/mL, whereas the greatest inhibitory effects were observed at concentrations of 1400 and 1800 mg/mL. The antibacterial activity of A. retroflexus extract may be associated with its antioxidant compounds, suggesting its potential for further investigation in pharmaceutical applications.

Keywords:

Antioxidant, Amaranthus retroflexus, Pseudomonas aeruginosa, Antibacterial activity, Total phenolic content

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Published

2026-08-02

Issue

Vol. 3 No. 4 (2026): Biocompounds (BIC)

Section

Articles

How to Cite

Moghadam, P. ., & Aryan, S. . . (2026). Assessment of Total Phenolic, Flavonoid, and Anthocyanin Contents of Amaranthus Retroflexus Extract and Its Inhibitory Effect on the Growth of Pseudomonas Aeruginosa. Biocompounds, 3(4), 234-244. https://doi.org/10.48313/bic.vi.80

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